| Outcome Measures: |
Primary: Clinical Findings: Plaque index score(PI), According to the tooth Plaque index, each tooth surface was divided into 4 separate regions and the plaque intensity for each region is scored as: 0 = No plaque in the gingival area. 1. = A film of plaque adhering to the free gingival margin and adjacent area of the tooth. The plaque may only be recognized by running a probe across the tooth surface. 2. = Moderate accumulation of soft deposits within the gingival pocket, on the gingival margin and/or adjacent tooth surface, which can be seen by the naked eye. 3. = Abundance of soft matter within the gingival pocket and/or on the gingival margin and adjacent tooth surface. Finally, scores are collected and calculate the arithmetic mean for the mouth score., Change from baseline(T0) to 3rd month(T1) and 6th month(T2)|Clinical Findings: Gingival index score (GI), The criteria are entirely confined to qualitative changes in the gingival soft tissue.Periodontal probe was used and gently inserted the sulcus. Each tooth surface was divided into 4 separate regions and the gingival bleeding activity for each region is scored as: 0 = Normal gingiva 1. = Mild inflammation -slight change in color, slight oedema. No bleeding on probing 2. = Moderate inflammation-redness, oedema and glazing. Bleeding on probing 3. =Severe inflammation -marked redness and oedema. Ulceration. Tendency to spontaneous bleeding. Finally, scores are collected and calculate the arithmetic mean for the mouth score., Change from baseline(T0) to 3rd month(T1) and 6th month(T2)|Clinical Findings: Bleeding on probing(BOP), Each tooth surface was divided into 4 separate regions and gently probed by using periodontal probe.There is a bleeding as a positive score, and there is no bleeding as a negative score. The BOP is considered positive if bleeding occurred between 30 seconds after probing. Whole the probed area and positive score area ratio were calculated and BOP(%)score was found., Change from baseline(T0) to 3rd month(T1) and 6th month(T2)|Clinical Findings:Probing pocket depth (PPD), Probing pocket depth (PPD) measured with a periodontal probe on 6 different points around each tooth. PPD is known as distance between gingival margin and the base of the pocket/sulcus. By using periodontal probe(Williams periodontal probe) 6 different points are measured. Each scores of surfaces are collected and averaged for the mouth score for PPD., Change from baseline(T0) to 3rd month (T1) and 6th month (T2)|Clinical Findings: Clinical Attachment Level (CAL), By using periodontal probe(Williams periodontal probe) 6 different points are measured.CAL, distance from the bottom of the pocket to the cemento-enamel junction of tooth. If there is gingival recession, recession depth is also added the CAL. By using periodontal probe(Williams periodontal probe) 6 different points are measured. Each scores of surfaces are collected and averaged for the mouth score for CAL., Change from baseline(T0) to 3rd month(T1) and 6th month(T2)|Biochemical Findings: RANKL (pg/ml), RANKL (pg/ml) were measured samples from gingival crevicular fluid by using commercial ELİSA kits according to the instructions of the kit manufacturer, Change from baseline(T0) to 3rd month(T1) and 6th month(T2)|Biochemical Findings: OPG (pg/ml), OPG (pg/ml) were measured samples from gingival crevicular fluid by using commercial ELİSA kits according to the instructions of the kit manufacturer, Change from baseline(T0) to 3rd month(T1) and 6th month(T2)|Biochemical Findings: MMP-8(ng/ml), MMP-8(ng/ml) were measured samples from gingival crevicular fluid by using commercial ELİSA kits according to the instructions of the kit manufacturer, Change from baseline(T0) to 3rd month(T1) and 6th month(T2)|Biochemical Findings: IL-beta(pg/ml), IL-beta(pg/ml) were measured samples from serum by using commercial ELİSA kits according to the instructions of the kit manufacturer, Change from baseline(T0) to 3rd month(T1) and 6th month(T2)|Biochemical Findings: RANKL/OPG, RANKL/OPG, numeric ratio that can be indicated as only numeric value(no need some kind of units), Change from baseline(T0) to 3rd month(T1) and 6th month(T2) |
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