| Outcome Measures: |
Primary: Metabolite- and lipid profiling, Metabolite- and lipid profiling of blood samples using metabolomics profiling platforms during euglycaemia, 5 minutes|Brain lactate concentration, Brain lactate concentration using non-invasive magnetic resonance (MR) spectroscopy during euglycaemia, 20 minutes|Brain adenosine triphosphate (ATP) concentration, Brain ATP concentration using non-invasive MR spectroscopy during euglycaemia, 20 minutes|Glycogen in muscle and adipose tissue, Glycogen in muscle and adipose tissue biopsies during euglycaemia, 5 minutes|Non-specific proteins in muscle and adipose tissue, Non-specific proteins in muscle and adipose tissue biopsies during euglycaemia, 5 minutes|Glycogen concentration, Glycogen in liver and muscle tissue using non-invasive MR spectroscopy during euglycaemia., 40 minutes | Secondary: Estimated glucose production during glucagon stimulation, Area under the curve (AUC) for plasma glucose during glucagon injections. Plasma glucose measurement, Every 5 minutes up to 5 hours|Estimated glucose production during epinephrine stimulation, Area under the curve (AUC) for plasma glucose during epinephrine infusion. Plasma glucose measurement, Every 5 minutes up to 90 minutes|Indirect calorimetry, Estimating resting metabolic rate, before and during hyperinsulinemic-hypoglycemic clamp, 60 minutes|Thermography, Estimating skin temperature, before and during hyperinsulinemic-hypoglycemic clamp, 5 minutes|Plasma lactate during glucagon injections., Plasma lactate during glucagon injections., Every 40 minutes up to 5 hours|Plasma free fatty acids during glucagon injections., Plasma free fatty acids during glucagon injections., Every 40 minutes up to 5 hours|Plasma glycerol during glucagon injections., Plasma glycerol during glucagon injections., Every 40 minutes up to 5 hours|Plasma alanine during glucagon injections., Plasma alanine during glucagon injections., Every 40 minutes up to 5 hours|Plasma β-hydroxybutyrate during glucagon injections., Plasma β-hydroxybutyrate during glucagon injections., Every 40 minutes up to 5 hours|Plasma insulin during glucagon injections., Plasma insulin during glucagon injections., Every 40 minutes up to 5 hours|Plasma glucagon during glucagon injections., Plasma glucagon during glucagon injections., Every 40 minutes up to 5 hours|Plasma metabolomics during glucagon injections., Plasma metabolomics during glucagon injections., Every 40 minutes up to 5 hours|Plasma lactate during epinephrine infusion, Plasma lactate during epinephrine infusion, Every 20 minutes up to 90 minutes|Plasma free fatty acids during epinephrine infusion, Plasma free fatty acids during epinephrine infusion, Every 20 minutes up to 90 minutes|Plasma glycerol during epinephrine infusion, Plasma glycerol during epinephrine infusion, Every 20 minutes up to 90 minutes|Plasma alanine during epinephrine infusion, Plasma alanine during epinephrine infusion, Every 20 minutes up to 90 minutes|Plasma β-hydroxybutyrate during epinephrine infusion, Plasma β-hydroxybutyrate during epinephrine infusion, Every 20 minutes up to 90 minutes|Plasma insulin during epinephrine infusion, Plasma insulin during epinephrine infusion, Every 20 minutes up to 90 minutes|Plasma glucagon during epinephrine infusion, Plasma glucagon during epinephrine infusion, Every 20 minutes up to 90 minutes|Plasma epinephrine during epinephrine infusion, Plasma catecholamines during epinephrine infusion, Every 20 minutes up to 90 minutes|Plasma norepinephrine during epinephrine infusion, Plasma catecholamines during epinephrine infusion, Every 20 minutes up to 90 minutes|Plasma metabolomics during epinephrine infusion, Plasma metabolomics during epinephrine infusion, Every 20 minutes up to 90 minutes|Personality traits using the psychometry questionnaire Type D Scale-14 (DS-14), Personality traits using the psychometry questionnaire DS-14, score between 0-28, the higher, the more likely they have type D personality, 30 minutes|Personality traits using the psychometry questionnaire Toronto Alexithymia Scale (TAS-20), Personality traits using the psychometry questionnaire TAS-20, score 20-100, the higher score the more likely they are alexithymia, 30 minutes|Diabetes and hypoglycaemia status using psychometry questionnaire Hypoglycemia Fear Survey - Worry (HFS-W), Diabetes and hypoglycaemia status using psychometry questionnaire HFS-W, score 0-72, the higher score the higher fear for hypoglycemia, 30 minutes|Diabetes and hypoglycaemia status using psychometry questionnaire Hypoglycemia Attitudes and Behavior Scale (HABS), Diabetes and hypoglycaemia status using psychometry questionnaire HABS, score from 14-45, higher score more fear of hypoglycemia, 30 minutes|Diabetes and hypoglycaemia status using psychometry questionnaire Problem Areas in Diabetes (PAID), Diabetes and hypoglycaemia status using psychometry questionnaire PAID, 0-80, the higher score, the more problems with diabetes, 30 minutes|Food consumption, Using Food Frequency Questionnaire to analyze food consumption, 30 minutes|Hypoglycemia awareness status, Using hypoglycemia awareness status questionnaire , 0-7, higher score indicate hypoglycemia unawareness, 10 minutes
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