| Outcome Measures: |
Primary: muscle oxidative stress in diabetic patients, muscular protein carbonylation level (unit: reactive carbonyl derivates, arbitrary unit) by Western blot (oxyblot kit from Chemicon) and pro et antioxidant genes expression (unit: mRNA levels normalized by housekeeping gene, arbitrary ratio) by real time polymerase chain reaction (RT-PCR), At 3 months of treatment | Secondary: - plasmatic oxidative stress by dosing plasmaticmarkers:, Dosing plasmatic malondialdéhyde, plasmatic H2O2, protein carbonylation of plasmatic protein and urinary isoprostans, and finally antioxidants (vitamins C and E, glutathione (unit: from µM to M), At 3 months of treatment|alterations in mitochondrial structure of skeletal muscle with transmission electron microscopy, Analysis of mitochondria area (in µm2) and density (in %), At 3 months of treatment|mitochondrial density by measuring the ratio mitochondrial Deoxyribonucleic acid (mtDNA)/nuclear DNA by real-time polymerase chain reaction (PCR) in skeletal muscle, At 3 months of treatment|mitochondrial function, Expression of genes implicated in mitochondrial action (messenger Ribonucleic acid (mRNA) levels by Reverse transcription polymerase chain reaction (RT-PCR) and proteins levels by Western Blot)(unit: arbitrary ratio relative to housekeeping gene/protein)., At 3 months of treatment|quantification of intramuscular lipids by histology (biopsy analysis), Staining Oil Red O evaluate the intramuscular lipid accumulation using the software ImageJ(unit: % of labelling by field)., At 3 months of treatment|sensitivity to insulin using a hyperinsulinemic euglycemic clamp, sensitivity to insulin will be expressed as the glucose infusion rate (GIR)/insulinemia ratio., At 3 months of treatment|uricemia and xanthine oxidase activity in sera and muscles(unit: mg/l for uricemia and mU/ml for XO activity), Plasma concentrations of uric acid will be measured before and after treatment to assess patient compliance . The reduction of xanthine oxidase activity in serum and muscle protein lysates will be measured using the kit " Amplex Red xanthine / xanthine oxidase assay kit" from Molecular Probes, At 3 months of treatment|Tolerance of the treatment measured by any adverse events during treatment and between each visit., Any adverse events during treatment and between each visit., during the 3 months of treatment
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