| Outcome Measures: |
Primary: Macrophage cell count and surface expression of markers of M1 and M2 polarization in blister fluid, To assess the effect of an 11 day course of once daily darapladib EC 160 mg on the number and phenotype (M1/M2 polarization) of macrophages isolated from blisters on subjects with type 2 diabetes mellitus (blisters induced by exposure to cantharidin for 48 hours). Biomarkers may include, but not be limited to: Cluster of differentiation (CD)11b, CD14, CD16, CD33, CD40, CD64, CD68, CD86, CD163, CD206, C-C chemokine receptor type 2 (CCR2), CX3 chemokine receptor 1 (CX3CR1), Up to 6 weeks | Secondary: Number of monocytes and surface expression of markers of M1 or M2 polarization in peripheral blood, To assess the effect of an 11 day course of once daily darapladib EC 160 mg on the number and phenotype of monocytes (M1/M2 ratio) isolated from peripheral blood of subjects with type 2 diabetes mellitus. Biomarkers may include, but not be limited to: CD11b, CD14, CD16, CD40, CD64, CD68, CD163, CD206, Up to 6 weeks|Enzymatic activity of Lp-PLA2 in blister fluid, Blood samples will be taken for assessments of enzymatic activity of Lp-PLA2 (measured by hydrolysis of radio-labeled platelet-activating factor \[PAF\]), Up to 6 weeks|Total cell count, lymphocyte count, neutrophil count and macrophage count in blister fluid, Blister fluids will be collected to assess the differences in blister phenotype (volume, appearance, cellularity) between subjects, Up to 6 weeks|Concentrations of soluble mediators of macrophage polarization, Soluble mediators will be measured in blister fluid assessed by a multiplex platform (Meso scale delivery or Luminex) and may include but not be restricted to matrix metalloproteinase 9 (MMP9), interleukin (IL)-12, IL-23, IL-10, interferon-gamma, tumor necrosis factor-alpha, IL-1 beta, IL-6, IL-8, IL-4, IL-13, transforming growth factor-beta, monocyte chemotactic protein-1 and C-reactive protein, Up to 6 weeks|Time to healing of cantharidin-induced blisters, Blisters will be considered to be healed when the blister cover has fallen off and the blister area becomes dry, Up to 8 weeks
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