| Outcome Measures: |
Primary: Glucagon Response to Acute Hypoglycaemia, Change in glucagon concentration from the initialisation phase to 40 minutes after occurrence of the autonomic reaction to hypoglycaemia, Change from initialisation phase to 40 minutes after onset of hypoglycaemia|Glucagon Response to Acute Hypoglycaemia, Area under the curve (AUC) from onset of the autonomic response to hypoglycaemia to 40 minutes after onset of the autonomic response. AUC values were calculated by the trapezoid method., 0, 10, 20 and 40 minutes | Secondary: Intact and Total Glucagon Like Peptide-1 (GLP-1), Intact and Total Gastric Inhibitory Peptide (GIP) Response to Acute Hypoglycaemia, Area under the curve (AUC) from onset of the autonomic response to hypoglycaemia to 40 minutes after onset of the autonomic response. AUC values were calculated by the trapezoid method., 0, 10, 20, 40 minutes|Epinephrine Response to Acute Hypoglycaemia, Area under the curve (AUC) from onset of the autonomic response to hypoglycaemia to 40 minutes after onset of the autonomic response. AUC values were calculated by the trapezoid method., 0, 10, 20, 40 minutes|Norepinephrine Response to Acute Hypoglycaemia, Area under the curve (AUC) from onset of the autonomic response to hypoglycaemia to 40 minutes after onset of the autonomic response. AUC values were calculated by the trapezoid method., 0, 10, 20, 40 minutes|Growth Hormone Response to Acute Hypoglycaemia, Area under the curve (AUC) from onset of the autonomic response to hypoglycaemia to 40 minutes after onset of the autonomic response. AUC values were calculated by the trapezoid method., 0, 10, 20, 40 minutes|Cortisol Response to Acute Hypoglycaemia, Area under the curve (AUC) from onset of the autonomic response to hypoglycaemia to 40 minutes after onset of the autonomic response. AUC values were calculated by the trapezoid method., 0, 10, 20, 40 minutes|Symptomatic Hormone Responses to Acute Hypoglycaemia., The symptomatic responses to hypoglycaemia were assessed using a standard validated symptom questionnaire adapted for experimental hypoglycaemia (McCrimmon et al (2003) Diabet.Med. 20: 507-509). A 7-point Likert scale (1=symptom absent; 7=symptom experienced with great intensity) was used to score presence and intensity of autonomic and neuroglycopenic symptoms of hypoglycaemia. Symptom scores were obtained during the initialisation phase, at occurrence of autonomic reaction and again 30 minutes later. For analyses the scale was considered as a continuous variable., Change from baseline symptomatic response at hypoglycaemia and 30 minutes after hypoglycaemia
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