| Outcome Measures: |
Primary: clinical attachment level, relative clinical attachment level (rCAL) was recorded at six sites per tooth with the aid of a computerized periodontal probe., baseline and two months | Secondary: Gene expression, Gingival tissue samples were obtained from periodontal sites (active or non-active) of the each patient in both groups during regular periodontal surgery. The samples are immediately submerged into liquid nitrogen to be then stored at -80º Celsius for RNA extraction and cDNA synthesis. The Real Time-PCR Array allowed simultaneous analysis of 84 genes involved in specific signaling pathways, including specific human inflammatory cytokines and receptors., Two months|Salivary proteins levels, Non-stimulated whole expectorated saliva was collected (\~ 3 ml) from each subject into sterile tubes and stored at -80º Celsius. The salivary inflammatory proteins levels were identified simultaneously using Multiplex Cytokine Profiling Assay, which allows the simultaneous detection of multiple analytes in saliva sample size., baseline and two months | Other: Probing pocket depth, It was recorded at six sites per tooth with the aid of a computerized periodontal probe., baseline and two months|Bleeding on probing, It was recorded at six sites per tooth with the aid of a computerized periodontal probe, and it was assessed according to presence or absence of bleeding up to 20 seconds after probing., baseline and two months|Plaque index, It was recorded at four sites per tooth to assess presence or absence of dental biofilm., baseline and two months|Glycated hemoglobin level, Metabolic control assessment, baseline and two months|Density loss, A complete series of radiographs was taken in each patient at baseline, using the paralleling technique. Two months after periodontal therapy, radiographs were taken with the same technique in teeth with active periodontal sites. The radiographs were digitized in tagged image file format (TIFF) on a scanner. Digital subtraction radiographs were performed with the baseline and 2-month radiographs using specific software. Changes between radiographs were depicted as a darkened area for loss of alveolar bone mass. These areas were measured (mm2) using specific measurements software., baseline and two months
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